Correction to: Journal of Investigative Dermatology Symposium Proceedings (2005) 10, 142–52. doi:10.111/j.1087-0024.2005.200405.x
In the publication by Kulesz-Martin et al., the authors reported that the TAp63α isoform was expressed in a keratinocyte model system in vitro, based upon reverse transcription-PCR analysis. We have since confirmed by immunoblotting using isoform-specific antibodies (provided by Dr Wendy Weinberg) and comparison with known molecular weight p63 isoforms ectopically expressed in keratinocytes that the TAp63α isoform is not expressed in the keratinocyte model but that the p63 isoforms present are identical to those in primary keratinocyte cultures. Thus, the p53 family members in keratinocytes are as follows in order of apparent molecular weight from greatest to least: ΔNp63α, TAp63β, TAp63γ, and either ΔNp63γ or ΔNp63s with ΔNp63α predominating. In the melanocyte model, the p63 isoforms expressed are TAp63γ, and either ΔNp63γ or ΔNp63s with the lower molecular weight isoform predominating.
The corrected Figures 2
(keratinocytes in left panel), and 5
(summary of changes in p53 family expressions during carcinogenesis of keratinocytes and melanocytes) are shown. All text in the paper referring to TAp63α
expression in keratinocytes and melanocytes should instead read ΔNp63α
and discussion of TAp63β
should read TAp63γ
, a more transcriptionally active p63 isoform.
© 2007 The Society for Investigative Dermatology, Inc. Published by Elsevier Inc.