WHAT miRNAs DO
- •miRNAs modulate transcription and repress translation, providing a level of control before the production of protein can exert biological effects.
- •A single miRNA can have multiple targets, potentially providing simultaneous regulation of many genes involved in a physiological pathway.
- •Multiple miRNAs with the same target reinforce the developmental program via redundant effectors of regulation.
- •Delivery: Because miRNAs do not freely diffuse into cells, special delivery approaches may be needed for both experimental and therapeutic design.
- •Instability: Small RNAs are unstable and may be degraded upon entering a cell.
- •Off-target effects: miRNA therapy can target an array of different gene products, with the possibility of undesired effects.
miRNA BIOGENESIS, PROCESSING, AND TARGET RECOGNITION
IDENTIFICATION OF miRNA TARGETS
RELEVANCE OF MIRNAS TO DERMATOLOGY
- 1.What is the typical length of miRNA nucleotides and its SEED sequence?
- A.22; 7.
- B.40; 22.
- C.7; 22.
- D.8; 7.
- 2.miRNA regulation involves which level(s) of control?
- A.DNA replication.
- B.mRNA destabilization.
- C.Protein repression.
- D.Both B and C.
- 3.Describe the mechanism of action of miRNAs.
- A.miRNAs use adapter proteins to interfere with replication.
- B.miRNAs cause destabilization of mRNA and inhibit translation initiation.
- C.miRNAs usurp the polymerase II machinery to decrease its activity and lower the levels of the endogenous mRNA.
- D.miRNAs rely on anti-microRNAs to potentiate their effects.
- 4.The best assay to identify whether a specific miRNA targets a particular transcript is which of the following?
- B.Western blot.
- D.Luciferase assay.
- 5.When a specific miRNA interacts with its target 3' UTR in a luciferase assay, the reporter signal
- C.Stays the same.
FUTURE POTENTIAL FOR MIRNAS IN DERMATOLOGY
- Supplementary Information
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The authors state no conflict of interest.
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