Advertisement
Journal of Investigative Dermatology Home
Advanced searchSave search
Original Article| Volume 104, ISSUE 4, P518-522, April 1995

Topical Tretinoin Increases the Tropoelastin and Fibronectin Content of Photoaged Hairless Mouse Skin

Open ArchiveDOI:https://doi.org/10.1111/1523-1747.ep12606007
Topical Tretinoin Increases the Tropoelastin and Fibronectin Content of Photoaged Hairless Mouse Skin
Previous ArticleIn Vivo Detection of Anthralin-Derived Free Radicals in the Skin of Hairless Mice by Low-Frequency Electron Paramagnetic Resonance Spectroscopy
Next ArticleInduction of Anagen in Telogen Mouse Skin by Topical Application of FK506, a Potent Immunosuppressant
      This paper is only available as a PDF. To read, Please Download here.
      Topical tretinoin treatment of photoaged hairless mice has been shown in previous studies to stimulate formation of a subepidermal zone of new connective tissue characterized by enhanced collagen synthesis. The aims of this study were to localize and/or quantify elastin, fibronectin, and glycosaminoglycans in the same model. Hairless mice (Skh-1) were irradiated thrice weekly for 10 weeks with gradually increasing doses of ultraviolet (up to 4.5 minimal erythema doses per exposure) from Westinghouse FS-40 bulbs. Mice were then treated five times a week with either 0.05% tretinoin, the ethanol:propylene glycol vehicle, or nothing for another 10 weeks. Controls included mice sacrificed after 10 weeks of ultraviolet treatment and age-matched untreated animals. The distribution of elastin and fibronectin was examined by immunofluorescence microscopy, which revealed fine fibrils In the subepidermal zone in tretinoin-treated skin. A quantitative slot-blot immunobinding assay showed that tretinoin induced a threefold higher amount of tropoelastin compared with controls. Insoluble elastin content (desmosine levels) was similar in all groups. Although fibronectin content was increased by ultraviolet radiation, tretinoin treatment induced the largest increase. In contrast, the amount of glycosaminoglycans, although increased by UVB radiation, was reduced by tretinoin treatment.

      Keywords

      References

        • Kligman L.H.
        • Akin F.J.
        • Kligman A.M.
        Prevention of ultraviolet damage to the dermis of hairless mice by sunscreens.
        J Invest Dermatol. 1982; 78: 181-189
        • Kligman L.H.
        • Akin F.J.
        • Kligman A.M.
        Sunscreens promote repair of ultraviolet radiation-induced dermal damage.
        J Invest Dermatol. 1983; 81: 98-101
        • Kligman L.H.
        • Chen H.D.
        • Kligman A.M.
        Topical retinoic acid enhances the repair of ultraviolet damaged dermal connective tissue.
        Connect Tissue Res. 1984; 12: 139-150
        • Kligman L.H.
        Effects of all-trans retinoic acid on the dermis of hairless mice.
        J Am Acad Dermatol. 1986; 15: 779-785
        • Bryce G.F.
        • Bogdan N.J.
        • Brown C.C.
        Retinoic acids promote the repair of the dermal damage and the effacement of wrinkles in the UVB-irradiated hairless mouse.
        J Invest Dermatol. 1988; 91: 175-180
        • Schwartz E.
        • Cruickshank F.A.
        • Mezick J.A.
        • Kligman L.H.
        Topical all-trans retinoic acid stimulates collagen synthesis in vivo.
        J Invest Demtatol. 1991; 96: 975-978
        • Chen S.
        • Kiss I.
        • Tramposch K.M.
        Effects of all-trans-retinoic acid on UVB-irradiated and non-irradiated hairless mouse skin.
        J Invest Dermatol. 1992; 98: 248-254
        • Kim H.J.
        • Bogdan N.J.
        • D'Agostaro L.S.
        • Gold L.I.
        • Bryce G.F.
        Effect topical retinoic acid on the levels of collagen mRNA during repair of UVB-induced dermal damage in the hairless mouse and the possible role of TGF-β as a mediator.
        J Invest Dermatol. 1992; 98: 359-363
        • Bissett D.L.
        • Hannon D.P.
        • Orr T.V.
        An animal model of solar aged skin. Histologic, physical and visible changes in chronically UV-irradiated hairless mouse skin.
        Photochem Photobiol. 1987; 46: 367-378
        • Fourtanier A.
        • Berrebi C.
        • Charles N.
        • Medaisko C.
        • Noblet J.P.
        Influence of UV light on natural skin aging in hairless mice.
        Cosmetic Dermatol. 1986; 1: 289-300
        • Sams W.M.
        • Smith J.G.
        The histochemistry of chronically sun damaged skin.
        J Invest Dermatol. 1964; 37: 447-452
        • Kligman A.M.
        Early destructive effects of sunlight on human skin.
        JAMA. 1969; 210: 2377-2380
        • Menter J.M.
        • Pearl W.S.
        • Yadven M.W.
        • Willis I.
        UV-induced elevation of skin glycosaminoglycan levels. I: Rapid measurement of skin glycosaminoglycans by titration with acridine orange.
        Photochem Photobiol. 1983; 38: 481-486
        • Schwartz E.
        Connective tissue alterations in the skin of ultraviolet irradiated hairless mice.
        J Invest Dermatol. 1988; 91: 158-161
        • Chen V.L.
        • Fleischmajer R.
        • Schwartz E.
        • Palaia M.
        • Timp1 R.
        Immunochemistry of elastotic material in sun damaged skin.
        J Invest Dermatol. 1986; 87: 334-337
        • Smith J.G.
        • Davidson E.A.
        • Taylor R.W.
        Human cutaneous mucopolysaccharides: the effects of age and chronic sun damage.
        in: Montagna W. Advances in Biology of Skin. Vol. 6, Aging. 1965: 211-218
        • Chatterjee R.
        • Benzinger M.J.
        • Ritter J.L.
        • Bissett D.L.
        Chronic ultraviolet B radiation-induced biochemical changes in the skin of hairless mice.
        Photochem Photobiol. 1990; 51: 91-97
        • Chen S.
        • Beehler B.
        • Sugimoto G.
        • Tramposch K.M.
        Effects of all-trans retinoic acid on glycosaminoglycan synthesis in photodamaged hairless mouse skin.
        J Invest Dermatol. 1993; 101: 237-239
        • Kligman A.M.
        • Grove G.L.
        • Hirose R.
        • Leyden J.J.
        Topical tretinoin for photoaged skin.
        J Am Acad Dermatol. 1986; 15: 836-839
        • Ellis C.N.
        • Weiss J.S.
        • Hamilton T.A.
        • Headington J.T.
        • Zelickson A.S.
        • Voorhees J.J.
        Sustained improvement with prolonged topical tretinoin (retinoic acid) for photoaged skin.
        J Am Acad Dermatol. 1990; 23: 629-637
        • Woodley D.T.
        • Zelickson A.J.
        • Briggaman R.A.
        • Hamilton T.A.
        • Weiss J.A.
        • Ellis C.N.
        • Voorhees J.J.
        Treatment of photoaged skin with topical tretinoin increases dermal-epidermal anchoring fibrils. A preliminary report.
        JAMA. 1990; 263: 3057-3059
        • Griffiths C.E.M.
        • Russman A.N.
        • Majmudar G.
        • Singer R.S.
        • Hamilton T.A.
        • Voorhees J.J.
        Restoration of collagen formation in photodamaged human skin by tretinoin (retinoic acid).
        N Engl J Med. 1993; 329: 530-535
        • Lunden A.
        • Berne B.
        • Michaelsson G.
        Topical retinoic acid treatment of photoaged skin: its effects on hyaluronan distribution in epidermis and on hyaluronan and retinoic acid in suction blister fluid.
        Acta Derm Venereol (Stockh). 1992; 72: 423-427
        • Varani J.
        • Nickoloff B.J.
        • Dixit V.M.
        • Mitra R.J.
        • Voorhees J.J.
        All-trans retinoic acid stimulates growth of adult human keratinocytes cultured in growth factor-deficient medium, inhibits production of thrombospondin and fibronectin, and reduces adhesion.
        J Invest Dermatol. 1989; 93: 449-454
        • Varani J.
        • Mitra R.S.
        • Gibbs D.
        • Phan S.H.
        • Dixit V.M.
        • Mitra Jr, R.
        • Wang T.
        • Siebert K.J.
        • Nickoloff B.J.
        • Voorhees J.J.
        All trans retinoic acid stimulates growth and extracellular matrix production in growth-inhibited cultured human skin fibroblasts.
        J Invest Dermatol. 1990; 94: 717-723
        • Varani J.
        • Astrom A.
        • Griffiths C.E.M.
        • Voorhees J.J.
        Induction of proliferation of growth inhibited keratinocytes and fibroblasts in monolayer culture by sodium lauryl sulfate: comparison with all-trans retinoic acid.
        J Invest Dermatol. 1991; 97: 917-921
        • Kligman L.H.
        Luna's technique–a beautiful stain for elastin.
        Am J Dermatopathol. 1981; 3: 199-201
        • Jahn R.
        • Schiebler W.
        • Greengard P.
        A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters.
        Proc Natl Acad Sci USA. 1984; 81: 1684-1687
        • Schwartz E.
        • Cruickshank F.A.
        • Lebwohl M.
        Determination of desmosines in elastin-related skin disorders by isocratic high performance liquid chromatography.
        Exp Mol Pathol. 1990; 52: 63-68
        • Schwartz E.
        • Mezick J.A.
        • Gendimenico G.J.
        • Kligman L.H.
        The in vivo prevention of corticosteroid-induced skin atrophy by tretinoin in the hairless mouse is accompanied by modulation of collagen, glycosaminoglycans, and fibronectin.
        J Invest Dermtol. 1994; 102: 241-246
        • Ross S.C.
        • Osborn T.G.
        • Dorner R.W.
        • Zucker J.
        Glycosaminoglycan content in the skin of the tight-skin mouse.
        Arthritis Rheum. 1983; 26: 653-657
        • Bitter T.
        • Muir H.M.
        A modified uronic acid carbazole reaction.
        Anal Biochem. 1962; 4: 330-334
        • Margelin D.
        • Fourtanier A.
        • Thevenin T.
        • Medaisko C.
        • Breton M.
        • Picard J.
        Alterations of proteoglycans in ultraviolet irradiated skin.
        Photochem Photobiol. 1993; 58: 211-218
        • Kligman L.H.
        • Mezick J.A.
        • Capetola R.J.
        • Thorne E.G.
        Lifetime topical application of tretinoin to hairless mice.
        Acta Derm Venereol (Stockh). 1992; 72: 418-422
        • Fisher G.J.
        • Tavakkol A.
        • Griffiths C.E.M.
        • Elder J.T.
        • Zhang Q.-Y.
        • Finkel L.
        • Danielpour D.
        • Glick A.B.
        • Highley H.
        • Ellingsworth L.
        • Voorhees J.J.
        Differential modulation of transforming growth factor-βl expression and mucin deposition by retinoic acid and sodium lauryl sulfate in human skin.
        J Invest Dermatol. 1992; 98: 102-108
        • Kligman L.H.
        The hairless mouse and photoaging: yearly review.
        Photochem Photobiol. 1991; 54: 1109-1118
        • Kligman A.M.
        • Graham G.F.
        Histological changes in facial skin after daily application of tretinoin for 5 to 6 years.
        J Derm Treatment. 1993; 4: 113-117

      Article info

      Publication history

      Accepted: December 27, 1994
      Received in revised form: December 17, 1994
      Received: August 10, 1994

      Identification

      DOI: https://doi.org/10.1111/1523-1747.ep12606007

      Copyright

      © 1995 The Society for Investigative Dermatology, Inc. Published by Elsevier Inc.

      User license

      Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0) |
      How you can reuse Information Icon
      Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0)

      Permitted

      For non-commercial purposes:

      • Read, print & download
      • Redistribute or republish the final article
      • Text & data mine
      • Translate the article (private use only, not for distribution)
      • Reuse portions or extracts from the article in other works

      Not Permitted

      • Sell or re-use for commercial purposes
      • Distribute translations or adaptations of the article

      Elsevier's open access license policy

      ScienceDirect

      Access this article on ScienceDirect

      The content on this site is intended for healthcare professionals.


      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the Cookie Preference Center for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties.


      RELX