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006 Topical application of caffeoyl prolyl histidine amide suppresses 2,4-dinitrochlorobenzene-induced atopic dermatitis-like symptoms in BALB/c mice

  • S. Jang
    Affiliations
    Laboratory of Cutaneous Aging and Hair Research, Seoul National University Clinical Research Institute, Seoul National University Hospital, Seoul, Jongno-gu, Korea (the Republic of)

    Institute of Human Environment Interface Biology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
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  • J. Ohn
    Affiliations
    Laboratory of Cutaneous Aging and Hair Research, Seoul National University Clinical Research Institute, Seoul National University Hospital, Seoul, Jongno-gu, Korea (the Republic of)

    Institute of Human Environment Interface Biology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)

    Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
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  • M. Park
    Affiliations
    Laboratory of Cutaneous Aging and Hair Research, Seoul National University Clinical Research Institute, Seoul National University Hospital, Seoul, Jongno-gu, Korea (the Republic of)

    Institute of Human Environment Interface Biology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
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  • O. Kwon
    Affiliations
    Laboratory of Cutaneous Aging and Hair Research, Seoul National University Clinical Research Institute, Seoul National University Hospital, Seoul, Jongno-gu, Korea (the Republic of)

    Institute of Human Environment Interface Biology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)

    Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
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  • K. Kim
    Affiliations
    Laboratory of Cutaneous Aging and Hair Research, Seoul National University Clinical Research Institute, Seoul National University Hospital, Seoul, Jongno-gu, Korea (the Republic of)

    Institute of Human Environment Interface Biology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)

    Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
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      The main components in atopic dermatitis (AD) pathogenesis are skin barrier abnormality, immunologic dysregulation, and pruritus. Considering the oxidative stress impacts on three main factors, we hypothesized antioxidant agents could be an effective treatment option in AD. To evaluate the effect of caffeoyl-prolyl-histidine amide (CA-L-Pro-L-His-NH2; CA-PH), a conjugated amide form of caffeic acid, 2,4-dinitrochlorobenzene (DNCB) was topically applied on the dorsal skin of BALB/c mice to induce AD-like symptoms and cutaneous lesions. CA-PH (0.5 mM and 5mM) or dexamethasone (25μg) was applied on the skin for two weeks to assess the relieving effects of DNCB-induced AD-like symptoms. CA-PH alleviated DNCB-induced AD-like symptoms quantified by dermatitis score, scratching behavior, and transepidermal water loss. Histopathological analysis revealed that CA-PH decreased DNCB-induced epidermal thickening, eosinophil and mast cell infiltration in dermis. We also found that CA-PH reduced serum IgE, and the mRNA levels of TSLP, interleukin (IL)-4, IL-25, IL-31, and IL-33 in the skin. This study showed that topical CA-PH could be a therapeutic option for AD.