246 Narcissus tazetta bulb extract delays cellular senescence by reducing mTOR pathway activation

      Somatic mammalian cells enter cellular senescence after a finite number of cell divisions. Senescent cells are characterized by their inability to proliferate and the secretion of promoters of inflammation and tissue deterioration. Telomere shortening is a main underlying mechanism of replicative senescence – the molecular clock counting down towards a programmed limit on cell replications, ending in senescence. This contributes to intrinsic tissue aging, leading to dryness, wrinkling, loss of elasticity, weakened barrier function, and hyperpigmentation. The bulb of Narcissus tazetta is a storage organ enabling the plant to survive adverse conditions through dormancy. When dormant, the plant produces reversible dormancy-inducing cell proliferation inhibitors (“dormins”).IFF/Lucas Meyer Cosmetics presents a natural aqueous extract of dormant Narcissus tazetta bulbs, capturing the plant dormancy concept for skin anti-aging. In vitro, treating a culture of aged human dermal fibroblasts with the extract showed: Restrained cell proliferation (by over 40%, from 0.01% extract); Telomere length preservation, significantly increasing telomere length in the aged cells; Significantly reduced activation of the mechanistic target of rapamycin (mTOR) signaling pathway, a central regulator of cellular senescence closely related to the DNA damage response, with influence on longevity and aging; Improved cellular function, significantly enhancing procollagen-1 production in aged cells. In a 28-day double-blind, placebo-controlled clinical study with 1% Narcissus tazetta bulb extract in formulation, the active significantly (p<0.05) improved key skin aging parameters, including: Wrinkles and lines (fine lines, wrinkle counts, wrinkle volumes); Skin elasticity; Skin pigmentation; and Skin barrier function (TEWL). These results demonstrate the value of Narcissus tazetta bulb extract as an anti-aging active ingredient through delayed cellular senescence.