Multiomic studies, including RNA sequencing, single-cell RNA sequencing, and epigenomics,
can provide insight into the connection between anatomically heterogeneous gene expression
profile of the skin and dermatoses-predisposed sites, in which RNA sequencing is essential.
Therefore, in this study, 159 skin samples collected mainly from discarded normal
skin tissue during surgical treatment for benign skin tumors were used for RNA sequencing.
On the basis of cluster analysis, the skin was divided into four regions, with each
region showing specific physiological characteristics through differentially expressed
gene analysis. The results showed that the head and neck region, perineum, and palmoplantar
area were closely associated with lipid metabolism, hormone metabolism, blood circulation,
and related neural regulation, respectively. Transcription factor enrichment indicated
that different regions were associated with the development of adjacent tissues. Specifically,
the head and neck region, trunk and extremities, perineum, and palmoplantar area were
associated with the central nervous, axial, urogenital, and vascular systems, respectively.
The results were imported into an open website (https://dermvis.github.io/) for retrieval. Our transcriptomic data elucidated that human skin exhibits transcriptomic
heterogeneity reflecting physiological and developmental variation at different anatomic
sites and provided guidance for further studies on skin development and dermatoses
predisposed sites.
Abbreviations:
DEG (differentially expressed gene), DETF (differentially expressed transcription factor), GO (gene ontology), HN (head and neck), PS (palms and soles), RNA-seq (RNA sequencing), TE (trunk and extremity), TF (transcription factor)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: September 16, 2022
Accepted:
August 26,
2022
Received in revised form:
August 5,
2022
Received:
March 18,
2022
accepted manuscript published online 17 September 2022; corrected proof published online 22 November 2022Identification
Copyright
© 2022 The Authors. Published by Elsevier, Inc. on behalf of the Society for Investigative Dermatology.
