August 2019 — Vol 139 No 8

Functional correction of COL7 and anchoring fibril formation at the dermal-epidermal junction.

A recurrent frameshift mutation inCOL7A1 was corrected in primary human RDEB fibroblasts using CRISPR/Cas9 based methodology. Injection of gene corrected fibroblasts into murine COL7 knockout mouse skin that had been grafted onto immunocompromised mice reduced epidermal-dermal cleft formation (top panels) inconjunction with deposition of human COL7 near the basement membrane zone ascompared to uninjected skin (remaining panels). Please see the Original Article byTakashima and coworkers on page 1711 for details.