September 2019 — Vol 139 No 9

Focal adhesions intercalate in hemidesmosomal chevrons in migrating keratinocytes and keratinocytes grown on X-shaped micropatterns.

Normal human keratinocytes were seeded onto fibronectin (FN)-coated X-micropatterns and fixed with paraformaldehyde one day later. Multiparameter fluorescence microscopy demonstrates that paxillin-positive focal adhesions (red)and ß4 integrin-containing hemidesmosomes (purple) occupy closely related but non-overlapping spaces. In this image, actin stress fibers are green and exclusively associate with focal adhesions and centrally located DAPI-stained nuclei are blue. See the Original Article by Pora and colleagues on page 1876 for the rest of the story.